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Immunofluorescence staining of HOPX (red; A and B), surfactant protein C (SFTPC) (green; A and B), smooth muscle action (ACTA2) (white; A–D), endomucin (EMCN) (red; C and D), and CSPG4 (green; C and D). CSPG4 is a pericyte marker. Lung images from E16.5 (A and C) and Postnatal Day 7 (B and D) mice are shown. Dramatic changes in peripheral lung architecture occur in the perinatal period.
