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. 2015 Sep 11;5:14078. doi: 10.1038/srep14078

Figure 3. Histochemical detection of ROS accumulation in leaves, and lipid peroxidation and loss of membrane integrity in roots of rice plants under co-application of NaHS and CdCl2.

Figure 3

(a) NBT staining of superoxide (O2•−), (b) DAB staining of hydrogen peroxide (H2O2), (c) Schiff’s test for lipid peroxidation and (d) Evan’s blue uptake test for loss of membrane integrity. Control, H2S, H2S + HT, Cd1, H2S + Cd1, Cd2, H2S + Cd2, and H2S + HT + Cd2 correspond to the group of seedlings exposed to only nutrients, 100 μM NaHS, 100 μM NaHS + 200 μM hypotaurine, 250 μM CdCl2, 100 μM NaHS + 250 μM CdCl2, 500 μM CdCl2, 100 μM NaHS + 500 μM CdCl2 and 100 μM NaHS + 200 μM hypotaurine + 500 μM CdCl2, respectively. NBT, nitroblue tetrazolium; DAB, diaminobenzidine.