Figure 7. Wfs1 is required for normal endoplasmic reticulum function in the prefrontal cortex.
Abundance of secreted polypeptides specifically enriched in the layer 2/3 pyramidal neurons is altered in the prefrontal cortex of Wfs1/CKO mice following ARS. (A) In the PFC lysates, WNT7a is significantly reduced in the Wfs1/CKO mice exposed to ARS (Two-way ANOVA—Bonferroni post hoc test: CKO Basal vs CKO + ARS, p < 0.001**, N = 3 per group). (B) proNTF3, the 23 kDa precursor of NTF3, is strongly elevated in Wfs1/CKO mice exposed to ARS indicating dysregulated precursor processing (Two-way ANOVA—Bonferroni post hoc test—CKO Basal vs CKO + ARS, p < 0.0001***, N = 3 per group). (C) BDNF, whose expression is not restricted to the layer 2/3 pyramidal cells in the prefrontal cortex, is not significantly altered. Abundance of the same polypeptides, WNT7a, proNTF3 and BDNF are not altered in the hippocampus (HPC) lysates regardless of the genotype and exposure to stress (D, E, F).