Schematic of the 48-well pedestal slide mold and pedestal slide assembly. (A) Measurements of mold are 75 mm long by 55 mm wide by 1 mm thick. The diameter of each hole is 7 mm. The width of the long border, short border, and width between holes is 1.5, 2.5, and 2.0 mm, respectively. (B) Flow chart of pedestal slide assembly as described in the text. A side view of a slice through the assembly sandwich is shown with the stainless steel molds in brown, empty holes in the mold in light gray, coverglass in black, agarose pedestals in dark gray, and culture samples in red.