TABLE 1.

History of 27 AHSV isolates from a 76-year period (1933–2009)

AHSV serotype	Original isolate	Isolate no. or yr of isolation	Passage levela	Origin	Accession no.
History of the current OIE reference strains of African horse sickness virus used at ARC-OVI
1	HS 61/61b	HS 29/62c	2S	Nelspruit, South Africa	KF859986 –KF859995
2	Unknown	HS 82/61c	3S	South Africa	KF859996–KF860005
3		HS 13/63c	4S	Malmesbury, South Africa	KM886354–KM886363
4	HS 47/58 (Specimen 341)	HS 32/62c	1S	Zimbabwe	KM609465–KM609474
5	FR (Fourie)	HS 30/62c	2S	South Africa	KM886344–KM886353
6	HS 09/58 (Mule 3858)	HS 39/63	1S	Kaalplaas, South Africa	KF860006–KF860015
6		HS 02/75c	4S	South Africa	KP009741–KP009750
7	HS 59/61	HS 31/62c	2S	Kaalplaas, South Africa	KF860016–KF860025
8		HS 10/62c	2S	Kenya	KF860026–KF860035
9		HS 90/61	4S	Chad (Fort Lamy)	KF860036–KF860045
History of recent field isolates of African horse sickness virus
1	HS 21/07		Unknown	South Africa	KP009621–KP009630
2	HS 90/07		2Vero	South Africa	KP009631–KP009640
3	HS 73/08		Unknown	South Africa	KP009641–KP009650
4	HS 128/06		1Vero	South Africa	KP009651–KP009660
5	HS 28/08		Unknown	South Africa	KP009661−KP009670
6	HS 04/08		Unknown	South Africa	KP009671−KP009680
7	HS 23/08		Unknown	South Africa	KP009681−KP009690
8	HS 29/00		1S,5BHK	South Africa	KP009691−KP009700
8	HS 83/04		3Vero,1BHK	South Africa	KP009721−KP009730
9	HS 27/08		Unknown	South Africa	KP009701−KP009710
9	HS 72/08		Unknown	South Africa	KP009731−KP009740
9	HS145/09		Unknown	South Africa	KP033466−KP033475
History of the historic neurotropic strains of African horse sickness virus
1	1180	1933	100+ in adult mice	South Africa	KP009711−KP009720
3	L	1940	100+ in adult mice	Ladysmith, South Africa	KP009761−KP009770
4	Vryheid	1938	100+ in adult mice	Vryheid, South Africa	KP009771−KP009780
5	Westerman	1936	100+ in adult mice	South Africa	KP009781−KP009790
7	Karen	1952	100+ in adult mice	Kabete, Kenya	KP009751−KP009760

^aThe passage level refers to the final passage number before dsRNA extraction and sequencing.

^bThe number after the / indicates the year of the original isolate and the number before the / indicates the number of the isolate during the particular year.

^cVirulent strains used to develop attenuated vaccines by serial passage in BHK-21 and selection of genetically stable large plaques on Vero cells (6, 7).