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. 2015 Jul 29;290(37):22460–22473. doi: 10.1074/jbc.M115.675595

FIGURE 4.

FIGURE 4.

ETV4 and ETV5 are involved in the proliferation of ES cells. A, proliferation of PE9 (WT) and PE15-2 (Etv4/5 dKO) ES cells. Either PE9 or PE15-2 ES cells were seeded at 500, 1000, or 2000 cells/well in a 96-well dish and subjected to a WST-1 assay. The results represent the means and S.D. (error bars) of triplicate assays. B, growth curves of PE9 and PE15-2 ES cells. PE9 and PE15-2 ES cells were cultured in the presence of LIF, and the cells were counted at the indicated days. The results represent the means and S.D. of four independent assays. C, direct cell counts of PE9 and PE15-2 ES cells in the 2i condition. PE9 and PE15-2 ES cells were cultured in the 2i condition, and the cells were counted at the indicated days. The results represent the means and S.D. of triplicate experiments. D, enhanced expression of cyclin-dependent kinase inhibitors in Etv4/5 dKO ES cells. Expression of cyclin-dependent kinase inhibitors, p16/p19, p15, and p57 mRNA in PE9 and PE15-2 ES cells was examined by RT-PCR. Gapdh was used as an internal control. The results are representative of three independent experiments.