FIGURE 5.

Etv4/5 dKO ES cells have impaired abilities to induce ectoderm marker gene expression. A, morphological signatures of EBs derived from PE9 (WT) and PE15-2 (Etv4/5 dKO) ES cells. PE9 and PE15-2 ES cells were cultured in suspension without LIF for 3–9 days. Morphological signatures were observed. Scale bar, 100 μm. B, expression of differentiation marker genes in PE9 and PE15-2 EBs. Expression of the indicated differentiation marker genes in the conditions of A in PE9 and PE15-2 EBs was examined by quantitative RT-PCR. Expression of Etv4 and Etv5 mRNA was only shown in PE9 ES cells. All samples were analyzed in triplicate, and the data were normalized to Gapdh expression. Error bars, S.D.