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. 2015 Jul 29;290(37):22460–22473. doi: 10.1074/jbc.M115.675595

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Several transcription factors are down-regulated in Etv4/5 dKO ES cells. A, GO term analysis of down-regulated candidates in Etv4/5 dKO ES cells. Genes involved in metabolic processes; regulation of transcription, DNA-dependent; translation elongation; transcription, DNA-dependent; and lipid biosynthetic are enriched significantly. GO categories are ranked according to their p value. B, expression of several transcription-related genes in PE9 and PE15-2 ES cells. Expression of transcription-related genes, including Tcf15, Gbx2, Lrh1, Zic3, and Baf60c, is down-regulated in PE15-2 ES cells, as shown by microarray analysis. Expression of the indicated genes in PE9 and PE15-2 ES cells was examined by quantitative RT-PCR. C, expression of several transcription-related genes in PE9 and PE15-2 ES cells in the 2i condition. PE9 and PE15-2 ES cells were cultured in the 2i condition, and expression of the indicated genes was examined by quantitative RT-PCR. All samples were analyzed in triplicate, and the data were normalized to Gapdh expression. Error bars, S.D.