FIGURE 2.

Formation and/or maintenance of a perinuclear concentrated network is compromised by loss of cysteine at position 4, 40, or 367 in K14. A, immunofluorescence imaging shows three modes of keratin network organization resulting from the expression of GFP-tagged K14WT, K14CF, C14C4A, K14C18A, K14C40A, K14C367A, and K14C389A in Krt14^−/− mouse keratinocytes in primary culture. Bar, 10 μm. B, population frequencies for each type of keratin IF network illustrated in A. Data are presented as average ± S.D. from three independent experiments. C, analysis of K14 WT and K14 cysteine variants by relating the percentage of cells with perinuclear concentrated networks (y axis) to those with pan-cytoplasmic networks (x axis). Each symbol represents one independent experiment; n = 3 experiments were performed per construct. Within here, the types of keratin filament organization promoted by the K14 variants being expressed are statistically the same. **, p < 0.01; ***, p < 0.001.