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Enzyme kinetics of Steap3 in detergent-activated membranes with FAD and Fe3+ substrates. FAD (A) and Fe3+-NTA (B) kinetics curves were generated from a nonlinear regression analysis with the Michaelis-Menten equation in GraphPad Prism 5.0. Error bars represent the S.E. from at least two measurements. Kinetic values in Tables 3 and 4 resulted from the mean of at least three independent experiments. fl., fluorescence.
