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. 2015 Jul 30;290(37):22649–22661. doi: 10.1074/jbc.M115.658468

FIGURE 5.

FIGURE 5.

HBXIP recruits p300 to transcription factor p53 to activate MDM2 promoter. A, P2 promoter activities of MDM2 were tested by luciferase reporter gene assays in MCF-7 cells. B, P2 promoter activities of MDM2 were examined by luciferase reporter gene assays in MCF-7 cells. C, P2 promoter activities of MDM2 were measured by luciferase reporter gene assays in MCF-7 cells. The efficiency of HBXIP siRNA was determined using Western blot analysis (WB). D, the interaction of p300 with p53 was detected by co-IP assays in MCF-7 cells. E, MCF-7 cells were transfected with HBXIP siRNA. ChIP assays were measured 24 h after transfection. F, the interference efficiency of p300 siRNA was tested in MCF-7 cells. G and H, the acetylation levels of histone H3 were examined by co-IP assays in MCF-7 cells with different treatments. Each experiment was repeated at least three times. Student's t test; *, p < 0.05; **, p < 0.01.