FIGURE 3.

HPAEC-PAD of glucose polymers from isoamylase-treated glycogen. Shown is a representative chromatogram where the signal from pulsed amperometric detection (PAD) in nC is recorded versus retention time in minutes of the resolved glucose polymers. The numbers associated with each peak correspond to the polymer length. Samples from non-exercised mice (black) and 1 h after exercise (gray) are shown. For quantitation as in Fig. 1F, the areas under the curve for each polyglucose species is integrated and expressed as a percentage of the sum of the signals (here from length 3 to 45 residues).