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. 2015 Jul 27;290(37):22686–22698. doi: 10.1074/jbc.M115.673897

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Glycogen branching in exercised Epm2a^+/+ and Epm2a^−/− mice. Glycogen branching was measured by iodine spectra (with absorbance normalized to 400 nm) as described under “Experimental Procedures” in samples from Epm2a^+/+ (filled symbols) and Epm2a^−/− (open symbols) mice. A, not run (NR, circles) and 3 h after exercise (triangles). B, not run (NR, circles) and 1 day after exercise (diamonds). C, not run (NR, circles) and 7 days after exercise (squares). D, branching was also monitored by HPAEC-PAD, which separated glucose polymers from 2 to 45 residues after isoamylase treatment. The percentage of chains longer than 15 residues is plotted as an index of the branching state of glycogen from wild type Epm2a^+/+ mice (filled bars) or Epm2a^−/− mice (open bars) under the indicated condition. Bars with the same letter are not significantly different from each other; different letters indicate p < 0.05.