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. 2015 Jul 15;290(37):22771–22781. doi: 10.1074/jbc.M114.629774

TABLE 2.

Antibodies for immunofluorescent staining

Primary antibody Secondary antibody 1:400
Anti-human TSG6 antibody (N-20;sc21828)1:100 Alexa 594 donkey anti-goat IgG antibody
Santa Cruz Biotechnology Molecular Probes A11058
Anti-mouse ITI-H2 antibody (K-17; sc21978)1:100 Alexa 594 or Alexa 488 donkey anti-goat IgG antibody
Santa Cruz Biotechnology Molecular Probes A11058 or A11055
Biotinylated anti-HABP antibody 1:200 Alexa 594 streptavidin
Seikagaku Biobusiness Corp. Molecular Probes S11227
Anti-mouseCD44 antibody (CL8944AP) 1:50 Alexa 594 goat anti-rat IgG antibody
Cedarlane Molecular Probes A11007
Anti-EGFP/GFP antibody (OSE00003G)1:100 Alexa 488 goat anti-rabbit IgG
Osenses Molecular Probes A11008
Anti-GFP antibody (GTX26673)1:100 Alexa 488 donkey anti-goat IgG antibody
GeneTex Molecular Probes A11055
Anti-heparan sulfate 10E4 1:100 Alexa 594 goat anti-mouse IgM antibody
Sekagaku Biobusiness Corp., A80120 Molecular Probes A21044
Anti-mouse perlecan HK102 1:50 Alexa 594 goat anti-rat IgG antibody
Sekagaku Biobusiness Corp., AY0116 Molecular Probes A11007
Anti-Pax7 antibody (NBP1-52374) 1:100 Alexa 594 donkey anti-goat IgG antibody
Novus Biologicals Molecular Probes A11058
Anti-fetal actin (Ac20.4.2) 1:10 Alexa 488 donkey anti-mouse IgG antibody
Progen Molecular Probes A21202 or
Alexa 488 goat anti-mouse IgG antibody
Molecular Probes A11029
Anti-skeletal actin (E3884) 1:200 Alexa 488 donkey anti-rabbit IgG antibody
Spring Bioscience Molecular Probes A21206