FIGURE 5.

ICMT deficiency increases RalB protein stability. A, RalB transcript levels were determined by TaqMan quantitative PCR analysis and normalized to GABPB1 in a triplicate set of assays ± S.E. Data displayed as relative to WT MEFs. B, endogenous RalB protein levels were quantified by densitometry of Western blot and normalized to total protein. Data are displayed as relative to WT MEFs. Experiment performed in triplicate. C, WT and Icmt^−/− MEFs were treated with 25 μg/ml of cycloheximide and lysed at the indicated time points. Lysates were probed by Western blot for total RalB, c-Myc, and for GAPDH to verify equivalent total protein. Protein levels from blots in panel were quantified by densitometry and normalized to 0 h.