Figure 3.
Extended L1 arrest reduces reproductive potential. (A) Egg-laying dynamics of individual animals scored in 24-hr intervals are plotted for D1, D8normal, and D8delayed. (B) Total brood size distributions are plotted for D1, D8unsorted, D8normal, and D8delayed (two to eight biological replicates are pooled). Box and whiskers plots show minimum, 25%, median, 75%, and maximum with outliers as dots. *P-value < 0.05, **P-value < 0.01; two-tailed t-test. (C) Proportion of individuals that were healthy and had laid eggs, had formed a bag of worms due to internal hatching, had laid no eggs, were dead, or were still L1–L3 larvae after 100 hr recovery is plotted (D1, n = 193; D8normal, n = 91; D8delayed, n = 274).