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. 2015 Jul 23;201(1):55–64. doi: 10.1534/genetics.115.178525

Figure 5.

Figure 5

Double coDel. (A) A schematic of the coDel experiment simultaneously targeting XRN2 and XRN4 loci. A part of the XRN2 locus and a part of the XRN4 locus were cloned into pMcoDel and introduced into the new MACs of the conjugating cells at 7 hpm. Deletions at the endogenous XRN2 and XRN4 loci in the MAC were analyzed by genomic PCR. (B and C) Results of genomic PCR detecting deletions at the endogenous XRN2 (B) and XRN4 (C) loci in the MAC. PCR products from the same progeny lines were separated in the same lane positions. Asterisks indicate progeny lines showing deletions at both XRN2 and XRN4 loci. The solid arrowheads indicate PCR products from the target loci without deletions. The open arrowheads show the expected positions of PCR products from the target loci with deletions corresponding to the exact target sequences.