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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biomaterials. 2015 Jul 21;67:354–364. doi: 10.1016/j.biomaterials.2015.07.039

Figure 3.

Figure 3

(A) After 7 days in culture, HEAVM co-cultures maintain valve cell phenotypes. VECs on the surface of the gel formed a confluent monolayer and express CD31 (green), while encapsulated cells had low levels of αSMA (scale bars = 50 μm). (B) HEAVM co-cultures, VEC only scaffolds, and VECs on TCPS all express significantly less αSMA mRNA relative to VIC only scaffolds. VICs cultured on TCPS express significantly more αSMA mRNA than all other groups. (C) All VEC based cultures have similar levels of CD31 gene expression, while VIC only scaffolds express significantly less CD31 than all other groups. *p<0.05 versus all others. ^p<0.05 versus all others. n=4