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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cytotherapy. 2013 Aug 29;15(12):1527–1540. doi: 10.1016/j.jcyt.2013.04.010

Figure 3. MSCs increase in forward and side scatter with increasing passage.

Figure 3

(A) FSC/SSC quadrant gating was applied to each individual dot plot at P3. The P3 gates were applied to P5 and P7 for each particular cell line. Changes in percent positive cells in the Upper Right Quadrant (URQ) were recorded, plotted, and grouped by cell line; n=1. Purple bar = P3; orange bar = P5; green bar = P7. (B) Example of quadrant gating analysis on FSC/SSC dot plots from donor 167696 at P3 (left), P5 (center) and P7 (right). Percent positive in URQ is shown in the red dashed line following gating analysis.