Figure 5. Percentage of Colony Forming Units (CFUs) tend to decrease with increasing passage, and varies between cell lines.
(A) CFUs were calculated as a percentage by quantifying the number of colonies, and normalizing to the number of plated cells at Day 0. Error bars represent standard deviation; n=3. Purple bar = P3; orange bar = P5; green bar = P7. Statistical comparisons were made within cell lines, relative to P3. (B) Statistical analysis of CFU data for comparison between cell lines. Purple shading = P3, orange shading = P5, green shading = P7. For (A) and (B), ***p<0.001, **p<0.01, *p<0.05, ns = not significant, n/a = not applicable.