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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Int J Parasitol. 2008 Jan 26;38(10):1083–1097. doi: 10.1016/j.ijpara.2008.01.002

Fig. 7.

Fig. 7

Expression of putative active domains of SET-domain protein representatives from Plasmodium falciparum and their histone lysine methyltransferase activities on recombinant histones. A) Expression and purification of P. falciparum histones shown in a Coomassie blue-stained 18% SDS-PAGE gel. M, molecular weight markers in kDa; H, equi-molar mixture of four recombinant histones. B) Western blot analysis of GST-fusion PfSET domains using the wheat germ expression system. C, empty vector control. C) Methylation assays of the in vitro expression products using recombinant P. falciparum histones. Upper panel indicates the Commassie blue-stained gel showing the positions of recombinant histones. Lower panel shows the fluorograph of the same gel after 1-month exposure. Numbers below the panels indicate the liquid scintillation counting data from 10 μl of the same reactions, where control (C) counting was arbitrarily set as the background value and subtracted from other reactions. D) Western blots of PfH4 methylation by recombinant His-tagged PfSET8 expressed from the wheat germ expression system using α-H4K20Me1, Me2 and Me3 antibodies. C, empty vector control; SAM, S-adenosyl methionine.