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. 2015 Jul 16;29(10):4145–4161. doi: 10.1096/fj.14-265462

Figure 1.

Figure 1.

Expression of Ol-epg5 during early development and in gonads of medaka. A) Expression of Ol-epg5 during early stages of medaka larval development. B) Expression of Ol-epg5 in adult tissues (male: upturned bars; female, downturned bars). C) BAC reporter expression of Ol-epg5 was detected as early as the morula stage. D–I) During medaka larval development the background expression of Ol-epg5 progressively became restricted to interneurons and sensory neurons on the respective dorsal and ventral sides of the notochord (D), brain (fore-, mid- and hind brain) (E, F), and PGCs (G–I) of the embryos. For better contrast, green fluorescence can be observed in the skin and eyes (dmrt1a green fluorescence) (D, F) and in pigment cells (F, auto-fluorescence). G–I) At stages 30–33, Ol-epg5 was specifically coexpressed with Ol-vas (a specific marker of the germ plasm) in the germ cells (arrowheads). J, K) At the hatching stage, Ol-epg5 was expressed in the male germ cells of the primordial gonad (J) but was absent in female germ cells (K). Sox9b fluorescence (green) allows visualization of the somatic presupporting cells surrounding the germ cells of the gonadal primordium (30). L–M) Ol-epg5 was highly expressed in the testes of adult medaka. Scale bars, 110 (C–F), 7.5 (G–K), and 3000 µm (L, M).