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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: J Neurochem. 2007 Nov 6;104(4):1116–1131. doi: 10.1111/j.1471-4159.2007.05039.x

Figure 6.

Figure 6

Carnosic acid (CA) accumulates both in neurons and in non-neuronal cells. (a, c, and e) Cortical cultures (E17, DIV21) treated with various concentrations of CAB for 20 h were stained with anti-MAP2 monoclonal antibody to identify neurons (green) and rhodamine-conjugated streptavidin antibody to detect intracellular CAB (red). Hoechst dye 33,258 was used to label nuclei (blue). (b, d, and f) Anti-S100 monoclonal antibody was used to identify non-neuronal cells (green). Yellow and white arrows indicate accumulation of CAB in non-neuronal cells and neurons, respectively. Scale bar: 25 μm.