Fig. 8.

ZmMYB94 gene structure and expression analysis. Southern analysis of fdl1-1 and wild-type alleles (A, B). DNAs from homozygous wild-type (+/+), heterozygous (+/-) and homozygous mutant (-/-) seedlings were cleaved with BamH1. Filters were hybridized with the En/Spm-specific probe (A) or the 78bp fragment flanking the En/Spm insertion (B). (C) Schematic representation of the ZmMYB94 gene showing the position of the three exons, indicated as grey rectangles, and the two introns, indicated as black lines. The En/Spm element inserted in the mutant allele is designated as a triangle, the 78bp genomic DNA flanking the insertion site, which was cloned after co-segregation analysis, is designed as a black rectangle and the region containing the MYB domain is highlighted in grey. The putative translational start (ATG) and stop (TAG) codons and polyA signal are reported. Exact positions are indicated with respect to the putative transcription initiation site. Names and positions of primers used for gene sequence analysis and qRT-PCR are also reported. The En/Spm element is not drawn to scale. (D) Relative expression level in different wild-type tissue samples from plants of the B73 inbred line. Expression level was established by qRT-PCR and the 18S gene was used as internal control. Bars represent means ±SD. DAP, days after pollination; cc, inside the closed coleoptile; co, emerging from the open coleoptile; i, immature. Tissue samples are listed in Supplementary Table S2.