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. 2015 Jun 23;66(19):5867–5880. doi: 10.1093/jxb/erv296

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Rescuing the hes embryo using culture media. (A) A germinated wild-type seed on ICM medium. (B) A seedling from a rescued wild-type embryo. (C) Callus induced from wild-type embryo and producing shoot under induction. (D) hes seeds on ICM medium, showing one seed germinated. (E) hes embryos have developed to form elongated hypocotyls and cotyledons. One embryo has a bulb-like structure possibly derived from the apex. (F) Callus from the hes embryo. Scale bars: 200 µm in A and B; 2mm in C-F.