Fig. 5.

Effect of oryzalin and taxol on CMT array and wall ingrowth papillae formation in adaxial epidermal cells of cultured V. faba cotyledons. Confocal images of CMTs immunolabelled with anti-α-tubulin and IgG–Alexa Fluor 488 conjugate (A, C, E) and SEM images of the cytoplasmic face of the outer periclinal wall to visualize wall ingrowth papillae (B, D, F) of epidermal cells of cotyledons cultured for 24h in MS medium alone (A, B) or 24h in the presence of, 20 µM oryzalin (C, D) or 5 µM taxol (E, F). In the presence of oryzalin, CMT arrays were depolymerized and then tubulin aggregated and exhibited depletion zones (arrowheads in C). In contrast, CMT arrays were stabilized in the presence of taxol (E) retaining an alignment comparable to those located in freshly harvested cotyledons (c.f. Fig. 2A). Bar, 10 µm.