Fig. 4.
Expression analysis of BPH29. (A) qRT-PCR analysis of the BPH29 expression pattern under BPH infestation. RBPH54 plants were collected 0, 24, 48, and 72h after infestation. Expression levels revealed that BPH29 is suppressed by BPH infestation. Student’s t-test: **P<0.01. Data are means ±SD (n=3 individuals). Expression levels are relative to OsActin1. (B) qRT-PCR analysis of BPH29 spatial- and temporal-specific expression. Different tissues from RBPH54 callus, three-leaf, tillering, and flowering stages were detected. BPH29 showed relatively high levels in callus and in roots at the three-leaf and flowering stages. Data are means ±SD (n = 3 individuals). Expression levels are relative to OsActin1 and log10 transformed. (C) Histochemical localization of BPH29 revealed by β-glucuronidase (GUS). The BPH29 promoter was fused with the GUS reporter gene (PBPH29::GUS), and introduced into RBPH54. GUS activity is indicated in blue. It showed that GUS stained the vascular system of leaf and root. Scale bar=1mm.