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Identification of novel microtubule-binding proteins (MBPs) through mass spectrometry analysis. (a) Schematic diagram for the preparation of MBP peptides by acetone precipitation and in-gel digestion. (b) The total ion chromatogram for nano liquid chromatography-mass spectrometry/liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis of MBP peptides. MS, mass spectrum; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
