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. 2015 Jul 3;4(9):1426–1431. doi: 10.1002/cam4.482

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© 2015 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of CD82 monoclonal antibody (CD82 mAb) on the mobilization of CD34⁺ cells. (A) FACS data. Gating of cell population. (B) Leukemia cells isolated from a patient with a World Health Organization (WHO) classification system subtype of minimally differentiated AML (case 1, 1 × 10⁶ cells) were intravenously injected into NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl/SzJ (NRG) mice (n = 4) via the tail vein. After 6 weeks, mice were treated with either CD82 mAb (1 μg) or isotype control IgG. Peripheral blood samples were collected at the indicated time point (0, 1, 3, 6 h) and stained with anti-human CD45 and CD34 antibodies. NRG mice (n = 4) without transplantation were used as negative control. *P < 0.05.