Extended Data Figure 6. Free food consumption is not reduced with repeated daily AGRP neuron photostimulation sessions.
a, Experimental design of free feeding experiment over repeated sessions. Three groups of AGRPChR2 mice were tested on a 15 session free feeding protocol (no lever pressing required) either under food restriction (black), ad libitum fed AGRP neuron photostimulated (cyan), or ad libitum fed without AGRP neuron photostimulation (grey) conditions. On each day, mice received one 2-hour session, where food was freely available for the first 40 minutes of the session. AGRP neuron photostimulated group received photostimulation for the entire 2 hour session (cyan). b, Food intake for each session of the free feeding experiment for food restricted (black, n=6), AGRP neuron 120-min-photostimulated (cyan, n=6), and no photostimulation (grey, n=6) groups. n.s. p>0.05. Values are means ± s.e.m.