Extended Data Figure 4. Lever pressing for food is sensitive to AGRP neuron photostimulation duration.
a, Experimental design of progressive ratio 7 lever-press experiment from Fig. 3 for a FR AGRP neuron photostimulated group and an AL-fed non-photostimulated group. The two additional groups of mice were trained to lever press in food-restriction on a PR7 reinforcement schedule. For the food-restricted with photostimulation group, mice were maintained on food-restricted and tested with PR7 reinforcement tests over 15 sessions with photostimulation. Each session was 2-h, where levers were available for the first 40 minutes of the session, and photostimulation was delivered for the length of the session (120 min, grey). Mice were then ad libitum re-fed and tested on a non-photostimulated PR7 session. For the AL-fed non-photostimulated group, mice were ad libitum re-fed following lever-press training and tested with PR7 reinforcement tests over 16 sessions, with no photostimulation delivered (beige). b, Lever presses for each PR7 session for FR AGRP neuron photostimulated mice (grey, n=11) mice and AL-fed non-photostimulated mice (beige, n=8). For comparison, data are shown for food-restricted and 120-min-photostimulated groups that are reproduced from Fig. 3b. c, Lever presses on first (1) and last (15) sessions in PR7 test for food-restricted with photostimulation mice (grey) mice and sated no photostimulation mice (beige). Also shown are data for food-restricted and 120-min-photostimulated groups that are reproduced from Fig. 3c. e, Experimental design of progressive ratio 7 lever-press experiment from Fig. 3 for a 40-min photostimulation group. One additional group of mice was trained to lever press in food-restriction on a PR7 reinforcement schedule. Mice were then ad libitum re-fed and tested with PR7 reinforcement tests over 15 sessions. Each session was 2-h, where levers were available for the first 40 minutes of the session, and photostimulation was delivered only while levers were available (grey). A non-photostimulated PR7 session was also performed after the 15th test session. f, Lever presses for each PR7 session for 40-min-photostimulated (grey, n=12) mice. Also shown are data for food-restricted and 120-min-photostimulated groups that are reproduced from Fig. 3b. g, Lever presses on first (1) and last (15) sessions in PR7 test for 40-min-photostimulated mice (grey). Also shown are data for food-restricted and 120-min-photostimulated groups that are reproduced from Fig. 3c. n.s. p>0.05, **p<0.01, ***p<0.001. Values are means ± s.e.m.