Effect of MBG on expansion of HPC and HSC in cord blood ex vivo. Panel A. Mononuclear cells were separated from cord blood from healthy infants (n = 4) enriched for CD34+ cells and cultured ex vivo in the presence or absence of MBG at the indicated doses. The effects of MBG on expansion of cell populations were determined after 4 days of culture followed by harvesting, staining with anti-human CD34, CD38, CD33 antibodies, and assessment by three-color flow cytometry. Data in Panel A show the CD34+CD33+D38- (HPC) cell population. Data in Panel B show the CD34+CD38-CXCR4+ (HSC) cell population from the same cultures as in Panel A. Data present mean cell numbers ± SD, * P< 0.05 vs. control with no MBG.