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. 2015 Sep 11;10(9):e0137745. doi: 10.1371/journal.pone.0137745

Fig 4. Osteogenic differentiation of OS cell lines.

Fig 4

(A) Alizarin red staining of cells derived from Trp53-heterozygous mice with either one Ptprz1 allele (+/-) or with Ptprz1-deficiency (-/-) reveals that both cell lines are able to form a mineralized matrix after 10 and 20 days of differentiation induced by ascorbic acid and ß-glycerophosphate. (B-D) qRT-PCR expression analysis shows that the differentiation is accompanied by increased expression of Bglap (encoding Osteocalcin), Ibsp (encoding Bone sialoprotein) and Phex. Values represent copy number (cn) relative to Gadph. Bars represent mean ± SD (n = 3). Asterisks indicate significant differences towards day 0 of corresponding genotype (p<0.05, Kruskal-Wallis followed by Dunn’s post-test).