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. 2015 Apr 15;2(2):ofv052. doi: 10.1093/ofid/ofv052

Figure 4.

Figure 4.

Age-associated changes in preexisting memory B cells. The number of H3N2 (H3)-specific A, immunoglobulin (Ig)G+ and B, IgA+ as well as H1N1 (H1)-specific C, IgG+ and D, IgA+ memory B cells were assessed by B cell enzyme-linked immunospot at day 0 before vaccination and on day 28 postvaccination. The day 0 geometric mean percent (GMP) of memory B cells of older adults were compared with middle-aged controls by generating day 0 GMP ratios (day 0 GMP of older adult group/day 0 GMP of 50–59 age group). A d0 GMP ratio less than 1 (dotted line) is indicative of fewer antigen-specific, preexisting memory B cells. The GMP ratio (day 28/day 0% antigen-specific cells) was calculated to determine changes in the number of memory B cells within each age group associated with vaccination. A GMP ratio of 1 (dotted line) is indicative of no change. Error bars represent 1 standard error. Significance is indicated by *P ≤ .05, **P ≤ .01, †P ≤ .001; n = 42.