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. 2015 Sep 4;9:5099–5113. doi: 10.2147/DDDT.S90201

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© 2015 Du et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Anthocyanins enhance cholesterol efflux and ABCA1 expression in HG-stimulated HK-2 cells.

Notes: Cells were preincubated with or without C3G and Cy (50 µM) for 1 h and then treated with 30 mM HG for 24 or 48 h. (A) Cholesterol mass inside the cell was measured by Oil Red staining. (B and C) Effect of C3G and Cy on cholesterol concentration in the medium (B) and intracellularly (C) was assayed by an Amplex Red Cholesterol Assay kit. (D) The expression levels of ABCA1 mRNA were analyzed by RT-qPCR. (E) The expression levels of ABCA1 protein were detected by Western blot. The values presented are the means ± SD of three independent experiments. *P<0.05, **P<0.01 versus control group, ^#P<0.05, ^##P<0.01 versus HG group.

Abbreviations: C3G, cyanidin-3-O-β-glucoside chloride; Cy, cyanidin chloride; h, hour/s; HG, high-glucose; NG, normal glucose; RT-qPCR, quantitative real-time polymerase chain reaction.