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. 2015 Sep 4;9:5099–5113. doi: 10.2147/DDDT.S90201

Figure 6.

Figure 6

Knockdown of LXRα abrogated the anthocyanins-induced ABCA1 gene expression and the cholesterol efflux in HK-2 cells.

Notes: Cells were transfected with pLV-NR1H3-shRNA plasmid or scrambled control shRNA plasmid for 24 h and then treated with HG (30 Mm) in the presence or absence of C3G and Cy (50 µM) for 48 h, respectively. (A) The effect of pLV-NR1H3-shRNA plasmid on LXRα expression was detected by Western blotting. (B) The expression levels of ABCA1 protein were detected by Western blot. (C) The expression levels of ABCA1 mRNA were analyzed by RT-qPCR. (D) Cholesterol concentration in cells was assayed by an Amplex Red Cholesterol Assay kit. The values presented are the mean ± SD of three independent experiments. *P<0.01 versus control group, #P<0.05 versus HG group. $P<0.05 versus HG + C3G group, ^P<0.01 versus HG + Cy group.

Abbreviations: C3G, cyanidin-3-O-β-glucoside chloride; Cy, cyanidin chloride; h, hour/s; HG, high-glucose; RT-qPCR, quantitative real-time polymerase chain reaction.