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. 2015 Sep 4;10:5647–5661. doi: 10.2147/IJN.S87307

Figure 9.

Figure 9

FITC-PAMAM or FITC-BC-PAMAM (green) colocalization with mitochondria (red) in (A) fibroblasts and (B) squamous cell carcinoma after 24 hours incubation with dendrimers (1–50 µM).

Notes: Mitochondria (red) were stained with MitoTracker® Deep Red FM and nuclei were stained with DAPI (blue). Colocalization is shown as a yellow signal. Cell imaging was performed on a Carl Zeiss Axio Observer Z1 LSM 710 inverted confocal microscope equipped with a 40× oil immersion objective.

Abbreviations: FITC-BC-PAMAM, fluorescein isothiocyanate-labeled biotin–polyamidoamine bioconjugate; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride.