Fig 3. Recombinant bCCL28 exhibits antimicrobial activity in vitro.
E. coli was incubated with 0.25 μM of mouse or bovine CCL28 or CCL27 for 2 hours. Percent survival was calculated using the following equation: (CFUpathogen + chemokine/CFUpathogen only) x 100. **p<0.01 as determined by Mann Whitney U test. Results are from three separate experiments, each performed in duplicate. Survival of bacteria in the CCL27 treated groups was scaled to 100% and the amount of killing in the CCL28 treated groups is shown as percent survival compared to the CCL27 treated group. Average bacterial survival seen in mCCL28 treated bacteria 28.3% (SE 1.35), survival observed in bCCL28 treated bacteria 30.3% (SE 1.29). Error bars represent standard error of the mean.