Fig 4. Recombinant bCCL28 has potent broad spectrum antimicrobial activity against mastitis causing bacteria.
Bovine CCL28 demonstrates dose dependent antimicrobial activity against Pseudomonas aeruginosa (A), methicillin-resistant Staphylococcus aureus (MRSA) (B), Streptococcus uberis (C), and Streptococcus agalactiae (D). Bacteria were incubated with varied doses of bCCL28 or bCCL27 for 1 hour. Percent survival was calculated using the following equation: (CFUpathogen + chemokine/CFUpathogen only) x 100. *p<0.05, **p<0.01 as determined by Mann Whitney U test. Results are from four or more separate experiments. Error bars represent standard error of the mean. Details on percent survival, standard error, and the number of times each experiment was performed are available in S1 Fig.