Figure 4. Analysis of marginal zone macrophages from Cnr2^tmZim mice.

(A) Splenocytes from WT (open bar) and Cnr2^tmZim (closed bar) mice were stained and analyzed for the percentage (left panel) and absolute number (right panel) of MARCO⁺ cells by flow cytometry. Data shown are the mean ± SEM of two independent experiments, each with three mice per group. (B) Seven µm frozen spleen sections from WT and Cnr2^tmZim mice were stained with anti-B220 (red) and anti-MARCO (green) to indicate B cell follicles (B220^hi, arrowheads) and MZ macrophages (MARCO⁺, thick arrows) residing in the MZ compartment outside the white pulp. Magnification is 100×. Data is representative of two independent experiments (n=2).