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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Semin Immunol. 2014 May 28;26(5):369–379. doi: 10.1016/j.smim.2014.04.002

Figure 5. CB2 is dispensable for the development/maintenance of lymph node and peritoneal cavity B cell subsets.

Figure 5

(A) Inguinal lymph node cells were stained with anti-B220, and analyzed by flow cytometry. The absolute cell numbers were determined by counting total LN cells with trypan blue. The percentage (left panel) and absolute numbers (right panel) of B220+ cells from WT (open bar) and Cnr2tmZim (closed bar) mice are indicated. Data are the mean ± SEM from 2 independent experiments (n = 6 mice/group). (B, C) Peritoneal cells were isolated from WT (open bar) and Cnr2tmZim (closed bar) mice and stained for B220, CD23, CD11b, and CD5 to identify total B (B220+), B2 (B220+CD23+), B1 (B220+CD23−CD11b+), B1a (B220+CD23−CD5+CD11b+) and B1b (B220+CD23−CD5CD11b+) cells. The percentage of total B and B2 and B1 cells among the B220+ population is shown (B). (C) The cumulative percentage (left panel) and absolute number (right panel) of B1a and B1b cells is shown. (B, C) Data are the mean ± SEM from 2 independent experiments (n=6 mice/group).

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