Skip to main content
. 2015 Sep 11;10(9):e0137917. doi: 10.1371/journal.pone.0137917

Fig 3. Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation.

Fig 3

TXL2 ALL cells, made maximally quiescent by culture in αMEM + 1% BSA for 24 hours without OP9 stromal support or FBS (marked ‘starved’ below panel), were stimulated by 20% serum addition and stroma at t = 0 (marked 'stimulated' below panel), while exposed (+) or not (no symbol) to 10 μM selumetinib. Phospho-flow using pErk1/2 (CST) in (A) left part of panel or pMek (BD) antibodies in (A) right part of panel taken at the indicated time points. (B) Western blot analysis for pErk1/2. One of two experiments with similar results. Gapdh, loading control. The membrane was sequentially stripped and reprobed with antibody.