(A) A phylogenetic tree of the WOPR proteins homologous to OsaA in several fungi. A. nidulans OsaA (AN6578), A. flavus (AFL2T_08419), A. fumigatus (Afu5g12960), A. clavatus (XP_001270095.1), A. niger (XP_001396650.1), H. capsulatum Ryp1 (ABX74945.1), F. oxysporum Sge1 (AGA55574.1), F. graminearum Fgp1 (I1S5P3), P. digitatum (EKV12249.1), P. chrysogenum (XP_002563706.1), P. oxalicum (EPS28657.1), P. marneffei (XP_002153295.1), F. fujikuroi (S0E3H0), F. verticillioides (W7MPI5.1), M. oryzae (XP_003713871.1), C. albicans Wor1 (Q5AP80) and S. pombe Pac2 (BAC54908.1). (B) AflWprA and OsaA polypeptides showing the amino acid similarity. Yellow box indicates the WOPR domain, NLS: nuclear localization sequence. (C) Colony photographs showing veA1 WT, osaA mutant (ΔosaA veA1) and cross-complementation (ΔosaA veA1 AflwprA) strains.