Table 2. The effect of metal cations and reagents (5 mM) on the activity of recombinant Egl7A.
Chemicals | Relative activity (%) a | Chemicals | Relative activity (%) |
---|---|---|---|
Control | 100 ± 1 | Co2+ | 90 ± 6 |
Fe3+ | 132 ± 6 | Cu2+ | 90 ± 5 |
Ca2+ | 119 ± 3 | Na+ | 65 ± 5 |
Cr3+ | 114 ± 4 | Pb2+ | 56 ± 3 |
K+ | 113 ± 2 | Mn2+ | 44 ± 6 |
Li+ | 111 ± 1 | Ag+ | 0 |
Zn2+ | 108 ± 4 | β-mercaptoethanol | 104 ± 2 |
Mg2+ | 95 ± 5 | EDTA | 76 ± 4 |
Ni2+ | 94 ± 4 | SDS | 5 ± 1 |
a Values represent the mean ± SD (n = 3) relative to untreated control sample