Fig 4. Modulation of PD-1 and GrzB protein co-expression during active TB disease and chemotherapy.

Frozen PBMCs from Pakistan were thawed and PD-1 and GrzB co-expression was investigated by flow cytometry. (A) The proportion of PD-1 cells which are also GzB positive in the CD3⁺CD8⁺ lymphocyte gate, CD3⁺CD8^- lymphocyte gate and CD3^- lymphocyte (NK cell) gate at diagnosis in TB patients (P: n = 18), latently infected contacts (C+: n = 7) and uninfected contacts (C-: n = 4). P values are derived from the Kruskal-Wallis test with Dunn’s multiple testing correction (B) Modulation of PD-1/GzB co-expression with the intensive phase of chemotherapy on CD3⁺CD8⁺ T cells, CD3⁺CD8^- lymphocytes and CD3^- lymphocytes (NK cells), analysed with the Wilcoxon signrank test. The bars show medians and the interquartile range.