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A) Individual pigment spectra (here, chl a, chl b, ββ-Car, Lut, c-Neo), and B) background basis spectra are assigned weights (C, D) in the NNLS regression, where the weights depend on the composition of the pigment extract. The weighted component spectra are summed to obtain the total pigment spectrum (E) and the total background spectrum (F). Adding together the pigment and background spectra yields the total spectrum of the extract (G).
