Skip to main content
. Author manuscript; available in PMC: 2016 Aug 25.
Published in final edited form as: Cell Rep. 2015 Aug 13;12(8):1314–1324. doi: 10.1016/j.celrep.2015.07.040

Figure 1. CD103+CD11b+ (DP) DCs are dispensable for commensal Th17 cell induction.

Figure 1

(A,B) CD11c+MHCII+ MNP subsets in SI LP of Langerin-DTA mice and control wildtype littermates (WT LM). (A left) FACS plots gated on CD11c+MHCII+ cells. A (right) Distribution of CD64 Mfs and CD11b SP DCs within the CD103−CD11b+ gate. Numbers represent percentage of cells in the corresponding gate. (B) Total number of cells in individual MNP subsets as defined in (A). (C,D) Total cell numbers and cell numbers in MNP subsets in the migratory DC fraction of mesenteric lymph nodes (MLN). Plots in (C) are gated on Lin−CD11cloMHCIIhi migratory DC. (E–G) Induction of RORγt+ Th17 cells by SFB in small intestinal (SI) LP. Plots gated on TCRβ+CD4+ cells. (H–J) Induction of IL-17+ Th17 cells by SFB in SI LP. Plots gated on TCRb+CD4+ cells. (K,L) Response of purified SI LP CD4 T cells to SFB antigens or control bacterial antigens (Jax antigens) prepared as described in Methods. Open circles in bar graphs in all panels represent data from individual animals. Data from three experiments with similar results.