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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jul 1;484:276–287. doi: 10.1016/j.virol.2015.06.014

Figure 1. Identification of redoxal and lomofungin as inhibitors of HIV-1 Vif-APOBEC3G interaction in a TR-FRET based assay.

Figure 1

A. TR-FRET based assay used to screen for inhibitors of HIV-1 Vif-A3G interaction. The interaction between purified GST-Vif (amino acids 1–94) and biotinylated A3G peptide (amino acids 110–148, a surrogate for the Vif binding site) was detected by Europium (Eu; donor fluorophore)-labeled anti-GST antibody and streptavidin-UL (Ulight; acceptor fluorophore). B. Dose response TR-FRET assay and counter screen testing of compounds redoxal and lomofungin for specificity and activity validation. The z-values calculated for the counter screens and cytotoxicity assays ranged from 0.70 to 0.91. C. Summary of redoxal and lomofungin including targets, known mechanisms of action, and structures.