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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jul 28;484:386–394. doi: 10.1016/j.virol.2015.06.018

Fig. 4. Sialic acid attachment enhances efficiency of viral gene expression.

Fig. 4

(A) ATCC L929 cells were adsorbed with 10 PFU/cell of T3SA+ or T3SA−. Increase in viral RNA levels was determined following incubation at 37°C for 6 h. The results are expressed as mean increase in RNA for three independent samples. Errors indicate SD. * P < 0.05 by student's t test in comparison to T3SA− at 0 h. ** P < 0.05 by student's t test in comparison to T3SA− at 6 h. (B) ATCC L929 cells were infected with T3SA+ or T3SA− at 10 PFU/cell. The results are expressed as the mean percentages of reovirus positive cells for three independent experiments. Error bars indicate SD. *, P < 0.05 by student's t test in comparison to T3SA+ at the comparable time point. ND, not determined (C) ATCC L929 cells pretreated with PBS or 40mU/mL neuraminidase at 37°C for 1 h, then washed and adsorbed with T3SA+ or T3SA− at 10 PFU/cell. Following incubation at 37°C for 18 h, reovirus positive cells were identified by indirect immunofluorescence. The results are expressed as the mean percentages of reovirus positive cells for three independent experiments. Error bars indicate SD. *, P < 0.05 by student's t test in comparison to T3SA+ in absence of neuraminidase.