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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Virology. 2015 Jul 1;484:288–304. doi: 10.1016/j.virol.2015.06.006

Figure 1. HeLa cells infected with Timer-CVB slowly change fluorescence from green to red.

Figure 1

The gene for “fluorescent timer” protein was inserted into the infectious plasmid clone for CVB. A) Upon infection with recombinant CVB3 expressing “fluorescent timer” protein (Timer-CVB), the slow conversion of the green fluorescing form of timer protein to red occurred over time in cells overlaid with agar. Initial sites of infection fluoresced red, while newly infected cells fluoresced green. B) HeLa cells infected with Timer-CVB (moi = 0.1) initially fluoresced green (recent viral protein) at 24 hours PI as determined by fluorescence microscopy. By 32 hours PI, both green and red fluorescence (matured viral protein) was observed in infected HeLa cells, and by 48 hours PI the majority of cells fluoresced brightly in the red channel. Fewer green and red infected cells were observed by fluorescence microscopy for HeLa cells treated with the antiviral drug ribavirin (100µg/ml) at every time point.